Cell Biology

Cellular Imaging Techniques for Neuroscience and Beyond by Floris G. Wouterlood

By Floris G. Wouterlood

The imaging of small mobile parts calls for robust tools, and a whole relatives of kit and methods according to the confocal precept has been built over the last 30 years. Such tools are common via neuroscience researchers, however the majority of those clients would not have a microscopy or a telephone biology backgrounds and do can stumble upon problems in acquiring and examining effects. This quantity brings specialists in high-resolution optical microscopy purposes in neuroscience and cellphone biology jointly to record the cutting-edge. Outlining what's at present attainable, the quantity additionally discusses promising advancements for the longer term and aids readers in settling on the main scientifically significant method of resolve their questions. every one bankruptcy discusses instrumentation and expertise in dating to software in learn. the entire universal and leading edge traits are lined - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, stay imaging, STED, PALM/STORM, etc.

  • Single and multiphoton confocal microscopy, and 4-pi confocal microscopy
  • Obtaining nanoresolution through photoactivation localization microscopy (PALM)
  • Several approaches that correlate observations in optical fluorescence microscopy and electron microscopy
  • Study of morphology and serve as through high-resolution fluorescence procedures
  • Additional high-resolution microscopic techniques

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